This program will predict what fragment sizes to expect when restricting genomic DNA with a selected restriction endonuclease. This will be based on published genome sequences, so unfortunately if your favourite organism has not been sequenced then using this program will not be possible. The following procedure should be followed: 1) Select a genome and an enzyme- press submit. 2) 'Hovering' the mouse cursor over the image will show the size and location of the fragments. Select a fragment in the left hand column and press submit query or click on a fragment on the virtual gel. 3) The next page will show all the genes that lie on that fragment. Click on one of these to show the info for the gene. Alternatively right click on the links below to download the sequence of the fragment or all the genes that are present on the fragment. Click to go to PFGE Predictor